Response Surface Optimization for Extraction of Phenolics from Coconut Testa (Pairings)
Keywords:
Coconut pairings, phenolics, antioxidant capacity, Coconut pairings, phenolics,response surface methodology
Abstract
Response surface methodology in conjunction with central composite design (CCD) was performed in the present study to optimize the extraction parameters for assessing maximum yield of total phenolic content and antioxidant capacity from coconut pairings. Solvent concentration (30-100%), extraction temperature (30-60°C) and extraction time (30-90 min) were used as the independent variables. The optimum conditions for extraction of total phenolics and antioxidant capacity from coconut pairings were found to be at ethanol concentration, 47.75% (v/v); extraction temperature, 49.84°C; and extraction time, 68.66 minutes. Under these optimal conditions, the model predicted a maximum response of 18.97 mg GAE/ g DW total phenolics and 38.09 mg AAE/g DW total antioxidant capacity of coconut pairing extracts and they were in close agreement with predicted values, thus indicating the suitability of the models developed and the success of RSM in optimizing the extraction conditions.
References
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Zhang, Z-S., Li, D., Wang, L.J., Ozkan, N., Chen, X.D., Mao, Z-H. & Yang, H-Z. (2007). Optimisation of ethanol-water extraction of lignans from flaxseed. Journal of Separation and Purification Technology, 57: 17-24.
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Appaiah, P., Sunil, L., Prasanth Kumar, P.K. and Gopala Krishna, A.G. (2014). Composition of Coconut Testa, Coconut Kernel and Its Oil. Journal of the American Oil Chemists’ Society. 91(6) : 917-924.
Chirinos, R., Rogez, H., Campos, D., Pedreschi, R. & Larondelle, Y. (2007). Optimisation of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruiz & Pavon) tubers. Journal of Separation and Purification Technology, 5: 217-225.
Gunathilake, K.D.P.P., Ranaweera, K.K.D.S., Rupasinghe, H.P.V., Perera, O.D.A.N., Jayaweera, H.P.S. (2017a). Response surface optimization of extraction of polyphenols and carotenoids from Sesbania grandiflora leaves with ethanol-water system. Asian Journal of Biotechnology and Bioresource Technology. 2(1).
Gunathilake, K.D.P.P., Rupasinghe H.P.V. (2014). Optimization of Water Based-extraction Methods for the Preparation of Bioactiverich Ginger Extract Using Response Surface Methodology. European Journal of Medicinal Plants. 4(8):893.
Gunathilake, K.D.P.P., Ranaweera, K.K.D.S., Rupasinghe, H.P.V. (2017b). Response surface optimization of phenolics and carotenoids extraction from leaves of Olax zeylanica. Annals. Food Science and Technology. 18(3)
Gunathilake KDPP, Ranaweera KKDS, Rupasinghe HPV, R. M. T. R. K. Rathnayaka. (2018). Optimizing the Extraction of Bioactive Compounds from Leaves of Gymnema Lactiferum, an Edible Green Leafy Vegetable. South Asian Research Journal of Natural Products. 1(2): 1-9.
Hayouni, E.A., Abedrabba, M., Bouix, M., Hamdi, M. (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L and Juniperus phoenicea L. fruit extracts. Food Chemistry. 105:1126–1134.
Hajj, Y.E., Louka, N., Nguyen, C., Maroun, R.G. (2012). Low-cost process for phenolic compounds extraction from cabernet sauvignon grapes (Vitis vinifera L. cv. cabernet sauvignon). Optimization by response surface methodology. Food Nutr. Science. 3:89–103.
Jamaluddin, A., Razak, D.L.A., Rashid, N.Y.A., Sharifudin, S.A., Kahar, A.Z.M., Saad, A.Z.M. and Long, K. (2016). effects of solid state fermentation by Monascus purpureus on phenolic content and biological activities of coconut testa and rice bran. Jurnal Teknologi, 78(11/12), pp.23-28.
Kong, K.W., Ismail, A.R., Tan, S.T., Prasad, K.M.N., Ismail A. (2010). Response surface optimisation for the extraction of phenolics and flavonoids from a pink guava puree industrial by-product. International Journal of Food Science and Technology 45:1739–1745
Oliveira, M. S., Feddern, V., Kupski, L., Cipolatti, E. P., Badiale-Furlong, E., and SouzaSoares, L. A. (2011). Changes in Lipid, Fatty Acids and Phospholipids Composition of Whole Rice Bran After Solid-state Fungal Fermentation. Bioresource Technology. 102: 8335–8338.
Prasad, K.N., Hassan, F.A., Yang, B., Kong, K.W., Ramanan, R.N., Azlan, A., Ismail, A. (2011). Response surface optimisation for the extraction of phenolic compounds and antioxidant capacities of underutilized Mangifera pajang Kosterm peels. Food Chemistry. 128(4):1121-1127.
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269, 337–341.
Richter, B.E., Jones, B.A., Ezzell, J.L., Porter, N.L., Avdalovic, N., Pohl, C. (1996). Accelerated solvent extraction: A technique for sample preparation. Analytical Chemistry. 68:033–1039.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of FC reagent. Methods in Enzymology, 29, 152–178.
Sies, H. and Stahl, W. (1995). Vitamins E and C, betacarotene, and other carotenoids as antioxidants. American Journal of Clinical Nutrition. 62:1315–1321.
Vita, J.A. (2005). Polyphenols and cardiovascular disease: Effects on endothelial and platelet function. American Journal of Clinical Nutrition. 81:292−297.
Zhao, X.L., Chen, W.J., Zhao, S.L. & Tang, M.M. (2012). Antioxidative activity of the extractives from coconut testa oil. Chinese Journal of Tropical Crops, 33, 162–165.
Zhang, Z-S., Li, D., Wang, L.J., Ozkan, N., Chen, X.D., Mao, Z-H. & Yang, H-Z. (2007). Optimisation of ethanol-water extraction of lignans from flaxseed. Journal of Separation and Purification Technology, 57: 17-24.
Zhang, Y., Zheng,Y., Duan, K. and Gui, Q. (2016). Preparation, antioxidant activity and protective effect of coconut testa oil extraction on oxidative damage to human albumin serum, International Journal of Food Science and Technology 2016, 51, 946–953.
Published
2018-04-01
How to Cite
Gunathilake, K. D. P. P., Dharmawansa, K. V. S. P., Weerasinghe, K. A. U. D., & Gimasha, A. J. N. (2018). Response Surface Optimization for Extraction of Phenolics from Coconut Testa (Pairings). CORD, 34(2), 20-29. https://doi.org/10.37833/cord.v34i2.16
Section
Articles
