Physicochemical Properties of Virgin Coconut Oil Extracted from Different Coconut (Cocos nucifera L) Varieties
Virgin coconut oil (VCO) is a superior edible oil extracted from fresh coconut (Cocos nucifera L) kernel using mixed coconut varieties without considering the varietal effect. Therefore, this research focuses on the quality evaluation of VCO extracted from four types of coconut varieties, namely Sri lanka Tall×Tall (TT), a tall variety of Gon Thambili (GT), a tall variety of Ran Thambili (RT) and Philippines tall variety of San Ramon (SR). Mature coconuts from each variety were collected from the Bandirippuwa Estate of the Coconut Research Institute, Sri Lanka to extract VCO by cold press oil extraction method. The extractability of VCO from different varieties was investigated. Moisture, free fatty acid (FFA), fatty acid profile (gas chromatography), peroxide value (PV), color (Lovibond scale), total phenolic substances (Galic acid equivalent), antioxidant capacity (α,α-diphenyl-β-picrylhydrazyl, 0.1mM – DPPH method) and sun protection factor (SPF) of VCO extracted from each variety were analyzed. The experiment was conducted as a completely randomized design with three replicates. Data were analyzed using ANOVA using Tukey’s test by MINITAB 17. Oil extractability (58%-59%), FFA (0.04%-0.12%), color (0.43–0.93) and fatty acid profile of VCO did not show variation among varieties. A higher concentration of total phenolic substances was observed in GT (0.24±0.03mg GAE/100g) while antioxidant capacity (857.19±14.99mg/ml) and SPF (8.99±1.26) was rich in RT.
AOAC International. (1999). Official Methods of Analysis of AOAC International, 17th Edition, Association of Analytical Communities, Gaithersburg.
AOCS. (1998). Method Ce 1-62. Preparation of fatty acid methyl ester. Official Methods and Recommended Practices of the American Oil Chemist’s Society, Champaign, IL, USA.
AOCS. (1998). Method Ce 2-66 Fatty acid analysis by gas chromatography.Official Methods and Recommended Practices of the American Oil Chemist´s Society, Champaign, IL, USA.
APCC. (2009). APCC standards for virgin coconut oil. Asian and Pacific Coconut Community.
Appaiah, P., Sunil, L., Prasanth Kumar, P. K., & Gopala Krishna, A. G. (2014). Composition of coconut testa, coconut kernel and its oil. Journal of the American Oil Chemists’ Society, 91(6), 917-924.
Bureau of Product Standards (BPS). (2004). Philippine National Standards: Virgin coconut oil. The Philippines, Department of Trade and Industry.
Carandang, E. V. (2008). Health Benefits of Virgin Coconut Oil. Indian coconut journal, 31(2), 8-12.
Chanchal, D. K., & Swarnlata, S. (2010). In vitro sun protection factor determination of herbal oils used in cosmetics. Pharmacognosy Research, 2(1), 22-25.
Che Man, Y. B., Abdul Karim, M. I. B., & Teng, C. T. (1997). Extraction of coconut oil with Lactobacillus plantarum 1041 IAM. Journal of the American Oil Chemists’ Society, 74, 1115–1119.
Choe, E., & Min, D. B. (2006). Mechanisms and factors for edible oil oxidation. Comprehensive reviews in food science and food safety, 5(4), 169-186.
Fernando, W. M. U. (1999). APCC-BUROTROP-COGENT. Project on the assessment of the performance of high yielding coconut varieties (HYCVS)/ hybrids and the varietal preferences of coconut farmers: a country report for Sri Lanka. Asia and Pacific Coconut Community, Yogyakarta, Indonesia.
German, J. B. & Dillard, C. J. (2004). Saturated Fats: What Dietary Intake?. American Journal of Clinical Nutrition, 80(3), 550-559.
Ghani, N. A. A., Channip, A. A., Chok Hwee, H. P., Ja’afar, F., Yasin, H. M., & Usman, A. (2018). Physicochemical properties, antioxidant capacities, and metal contents of virgin coconut oil produced by wet and dry processes. Food Science & Nutrition, 6(5), 1298–1306.
Henna Lu, F. S., & Tan, P. P. (2009). A comparative study of storage stability in virgin coconut oil and extra virgin olive oil upon thermal treatment. Int Food Res J, 16, 343–354.
Lister, E., & Wilson, P. (2001). Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication). Crop Research Institute, Lincoln, New Zealand, 7, 235-239.
Liyanage, D. V. (1958). Varieties and forms of the coconut palm grown in Ceylon. Ceylon Coconut Quarterly, 9, 1–10.
Man, Y. B. C., & Manaf, M. A. (2006). Medium-chain triacylglycerols. In Nutraceutical and specialty lipids and their co-products (pp. 41-70). CRC Press.
Mansor, T., Che Man, Y., Shuhaimi, M., Abdul Afiq, M., & Ku Nurul, F. (2012). Physicochemical properties of virgin coconut oil extracted from different processing methods. Int. Food Res. J., 19, 837–845.
Marasinghe, S. S. K., Marikkar, J. N., Yalegama, C., Wimalasiri, S., Seneviratne, P. R. G., Weerasooriya, R., & Liyanage, R. (2019). Comparison of inter varietal differences in chemical composition and nutritional properties of coconut testa flour. Journal of the National Science Foundation of Sri Lanka, 47(3).
Marina, A. M., Che Man, Y. B., Nazimah, S. A. H., & Amin, I. (2008). Antioxidant capacity and phenolic acids of virgin coconut oil. International journal of food sciences and nutrition, 60, 1–10.
Marina, A. M., Che Man, Y. B., Nazimah, S. A. H., & Amin, I. (2009). Chemical properties of virgin coconut oil. Journal of the American Oil Chemists’ Society, 86(4), 301-307.
Ramesh, S. V., Pandiselvam, R., Thushara, R., Manikantan, M. R., Hebbar, K. B., Beegum, S., ... & Shil, S. (2020). Engineering intervention for production of virgin coconut oil by hot process and multivariate analysis of quality attributes of virgin coconut oil extracted by various methods. Journal of Food Process Engineering, 43(6), e13395.
Rupasinghe, W. T. D. B., Yalegama, L. L. W. C., Arachchi, L. V., & Gunarathne, D. M. A. (2013). Characterization of coconut oil in different varieties in Sri Lanka. Tropical Agriculture, 90, 205-216.
Sayre, R. M., Agin, P. P., LeVee, G. J., & Marlowe, E. (1979). A comparison of in vivo and in vitro testing of sunscreening formulas. Photochemistry and Photobiology, 29(3), 559-566.
Seneviratne, K. N., & Jayathilaka, N. (2016). Coconut oil: chemistry and nutrition. Battaramulla: Lakva Publisher.
Seneviratne, K. N., & Sudarshana Dissanayake, D. M. (2008). Variation of phenolic content in coconut oil extracted by two conventional methods. International journal of food science & technology, 43(4), 597-602.
Seneviratne, K. N., HapuarachchI, C. D., & Ekanayake, S. (2009). Comparison of the phenolic-dependent antioxidant properties of coconut oil extracted under cold and hot conditions. Food Chemistry, 114(4), 1444-1449.
Senphan, T., & Benjakul, S. (2016). Chemical compositions and properties of virgin coconut oil extracted using protease from hepatopancreas of Pacific white shrimp. European Journal of Lipid Science and Technology, 118(5), 761-769.
Shimamura, T., Sumikura, Y., Yamazaki, T., Tada, A., Kashiwagi, T., Ishikawa, H., ... & Ukeda, H. (2014). Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives–inter-laboratory evaluation study–. Analytical Sciences, 30(7), 717-721.
SLSI, Sri Lanka Standards Specification for coconut oil. (2012). SLS 32: 2012. Sri Lanka Standards Institution, 17, Victoria Place, Elvitigala Mawatha, Colombo 8, Sri Lanka.
SLSI, Sri Lanka Standards Specification for coconut oil. (2017). SLSI 32: 2017.Sri Lanka Standards Institution, 17, Victoria Place, Elvitigala Mawatha, Colombo 8, Sri Lanka.