Biogenic Nanosilver Mediated by Coir, Medicinal Plant Extracts and their Antimicrobial Validation
Keywords:
Green synthesis, Silver nanoparticles, Plant extracts, Antimicrobial activity
Abstract
Knowing the magnitude of therapeutic plants such as Micrococca mercurialis (MM), Abutilon palmeri (AP) and Callistemon citrinus (CC), we also planned to extend our research work on Cocusnucifera fibers (CF) and dust (CD) as it has copious medicinal properties. The current study deals with the green synthesis of silver nanoparticles (AgNPs) from fresh aqueous extracts and AgNO3 solution. The biogenic conversion of silver ion to silver is relatively expeditious at two different time intervals and pH. The isolated silver nanoparticles (AgNPs) from the bio extracts were identified initially by scrutinizing the colour variations. The biosynthesized AgNPs were characterized by UV, XRD, Laser Diffraction Particle size Analyser, fluorescence spectroscopy, SEM and TEM. UV absorbance at 435-460nm for silver nanoparticles was observed for the above extracts. The XRD pattern of all extracts showed the distinctive Bragg peaks of face centre cubic (fcc) crystalline system available in nature. SEM and TEM analysis of the silver nanoparticles indicated that the particle size was in the range of 2-100nm with polygonal and spherical shapes. The biosynthesized AgNPs were evaluated for antimicrobial activities. The CFAgNPs showed an efficient antibacterial activity at lower concentration (25mg/ml) against Pseudomonas demolytica followed by Staphylococcus aureus and Escherichia coli. Similarly all plant extracts have better activity against fungal strains. It is concluded that the biogenic blend of AgNPs is simple, extremely rapid, beneficial, eco-friendly and more stable without any toxic effects. Of these, CFAgNps may be used for the preparation of antibacterial groupings against Pseudomonas demolytica and Staphylococcus aureus.
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Alarcon, E.I., Udekwu, K., Skog, M., Pacioni, N.L., Stamplecoskie, K.G., González-Béjar M. 2012. The Biocompatibility and Antibacterial Properties of Collagen-Stabilized, Photo chemically Prepared Silver Nanoparticles. Biomaterials, Vol 33(19) 4947-4956.
Ankanna, S., Prasad, T.N.V.K.V., Elumala, i E.K., Savithramma, N. 2010. Production of biogenic silver nanoparticles using Boswelliaovalifoliolata stem bark. Dig. J. Nanomater. Biostruct, Vol 5(2) 369-72.
Ashokkumar, S., Ravi, S., Kathiravan,V., Velmurugan, S. 2015. Synthesis of silver nanoparticles using A. Indicum leaf extract and their Antibacterial Activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol 134 (2015) 34-39.
Awwad, A.M., Salem, N.M., Abdeen, A. 2012. Biosynthesis of silver nanoparticles using Oleaeuropaea leaves extract and its antibacterial activity. Nanosci Nanotechno,Vol 2(6) 164-170.
Chen, P., Song, L.Y., Liu, Y.K. 2007. Synthesis of Silver Nanoparticles by Gamma-Ray Irradiation in Acetic Water Solution Containing Chitosan. Radiation Physics and Chemistry, Vol 76 (7) 1165-1168.
Christensen, L., Vivekanandhan, S., Misra, M., Mohanty, A.K. 2011. Biosynthesis of silver nanoparticles using murrayakoenigii (curry leaf): an investigation on the effect of broth concentration in reduction mechanism and particle size. Adv Mater Letters, Vol 2(6) 429-434.
Datt, S.,Vandana, R., Shivaraj, N., Jyothi, H., Ashish, K.S., Jasmine, M. 2014. Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (Turmeric) and application studies against MDR E. coli and S. aureus. Bioinorg Chem Appl, Vol 2014 (2014) Article ID 408021, 8 pages.
Dwivedi, A.D., Gopal, K. 2010. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloid Surf A Physicochem Eng Aspect, Vol 369 (2010) 27-33.
Emerich and Thanos. 2007. Targeted nano particle- based drug delivery and diagnosis. J Drug Target, Vol 15(3) 163-83.
Fox, C.L., Modak, S.M .1974. Mechanism of silver sulfadiazine action on burn wound infections. Antimicrob Agents Chemother, Vol 5(6) 582-588.
Galib, B.M., Mashru, M., Jagtap, C., Patgiri, B.J., Prajapati, P.K. 2011. Therapeutic Potentials of Metals in Ancient India: A Review through Charaka Samhita. Journal of Ayurveda and Integrative Medicine, Vol 2(2) 55-63.
Haytham, M.M., Ibrahim. 2015. Green synthesis and characterization of silver nano particles using Banana peel extracts and their antimicrobial activity against representative micro organism, Journal of Radiation research and applied science, Vol 8(3) 265-275.
Husen, A., Siddiq, K.S. 2014. Phytosynthesis of nanoparticles: concept, controversy and application. Nanoscale research letters, Vol 9(1) 229.
Husen, A., Siddiqi, K.S. 2014. Carbon and fullerene nanomaterials in plant system. J Nanobiotechnol, Vol 12(1) 2-10
Joerge, T.K., Joerger, R., Olsso, E., Granqvist, C.G. 2001. Bacteria as workers in the living factory: Metal-accumulating bacteria and their potential for materials science. Trends Biotechnol, Vol 19(1) 15-20.
Khalil, M.M.H. 2014. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arab J Chem, Vol 7(6) 1131-1139.
Khan, A., El-Ton, A.M., Alrokayan, S., Alsalhi, M., Alhoshan, M., Aldwayyan, A.S. 2011. Microwave-Assisted Synthesis of Silver Nanoparticles Using Poly-N Isopropyl Acrylamide/Acrylic Acid Microgel Particles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol 377 (2011) 356-360.
Krishnaraj, C., Jagan, E.G., Rajasekar, S., Selvakuma, P., Kalaichelva, P.T., Mohan, N. 2010. Synthesis of silver nanoparticles using Acalyphaindica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B Biointerfaces, Vol 76(1) 50-56.
Krishnaraj, C., Jagan, E.G., Rajasekar, S., Selvakumar, P., Kalaichelvan, P.T., Mohan, N. 2010 Synthesis of silver nanoparticles using Acalyphaindica leaf extracts and its antimicrobial activity against water borne pathogens. Colloids Surf B Biointerfaces, Vol 76(1) 50-56.
Krithiga, N., Rajalakshmi, A., Jayachitra, A. 2015. Green Synthesis of Silver Nanoparticles Using Leaf Extracts of Clitoriaternatea and Solanumnigrum and Study of Its Antibacterial Effect against Common Nosocomial Pathogens. Journal of Nanoscience, Vol 2015 (2015): 8
Logeswari, P, Silambarasan, S., Abraham, J. 2012. Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc, Vol 19(3) 311-317.
Lu, L., Sun, R.W.Y., Chen, R., Hui, C., Ho, C.M., Luk, J.M. 2008. Silver nanoparticles inhibit hepatitis B virus replication. Antivir Ther, Vol 13(2) 253-262.
Lu, Z., Dai, T., Huang, L., Kurup, D.B., Tegos, G.P., Jahnke, A., Wharton, T., Hamblin. 2010. Photodynamic therapy with a cationic functionalized fullerene rescues mice from fatal wound infections. Nanomedicine, Vol 5(10) 1525-1533.
Manisha Deb Mandal, Shyamapada Mandal. 2011. Coconut (Cocos nucifera L.: Arecaceae): In health promotion and disease prevention Asian Pacific Journal of Tropical Medicine, Vol 4(3 )241-247.
Manisha Debmandal, Shyamapada Mandal. 2010. Coconut (Cocos nucifera L.: Arecaceae): In promotion and disease prevention. Asian Pacific Journal of Tropical Medicine, Vol 241-247.
Mohammadinejad, R., Karimi, S., Iravani, S., Varma, R.S. 2016. Plant-derived nanostructures: types and applications. Green Chemistry, Vol 18(1) 20-52.
Mostafa Khalil, M.H., Eman, H. Ismail, Fatma El-Magdoub, 2012. Biosynthesis of Au nanoparticles using olive leaf extract: 1st Nano Updates Arabian Journal of Chemistry, Vol 5(4) 431-437.
Nabikhan, A., Kandasamy, K., Raj, A., Alikunhi, N.M. 2010. Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from salt marsh Sesuviumportulacastrum L. Colloids Surf. B, Vol 79(2) 488-493.
Nadkarni, K.M.1908. Indian plants and drugs. Popular prakashan, Dewey number 615. 1154, ID 3676306.
Padalia, H., Moteriya, P., Chanda, S. 2014. Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential. Arab J Chem, Vol 8(5) 732-741.
Parasad, K.S., Pathak, D., Patel, A., Dalwadi, P., Prasad, R., Patel, P., Selvaraj, K. 2011. Biogenic synthesis of silver nanoparticles using Nicotianatobaccum leaf extract and study of their antimicrobial effect. Afr J Biotechnol, Vol 10(41) pp. 8122-8130.
Rai, M., Ingle, A. 2012. Role of nanotechnology in agriculture with special reference to management of insect pests. Appl Microbiol Biotechnol, Vol 94(2) 287-293.
Published
2017-10-01
How to Cite
Kanagaraju, G., Sebastian, S., & Ravindranath, A. D. (2017). Biogenic Nanosilver Mediated by Coir, Medicinal Plant Extracts and their Antimicrobial Validation. CORD, 33(2), 35-53. https://doi.org/10.37833/cord.v33i2.50
Section
Articles
