AN IMPROVED METHOD FOR ISOLATION OF THIELAVIOPSIS PARADOXA FROM STEM BLEEDING AFFECTED COCONUT PALMS

  • Anil Kumar
  • K.K.N. Nambiar
Keywords: Stem Bleeding, Coconut Palms

Abstract

Stem bleeding of coconut caused by Thielaviopsis paradoxa (de Seynes) Von Hohnel is an important disease affecting coconut in many countries (Menon and Pandalai, 1958; Ohler, 1966, Nambiar and Sastry, 1988). lsolation of the pathogen from diseased tissues on different agar based media has given inconsistent results (Anon., 1976; Anon., 1986), None of the selective media reported for certain related fungi viz., T. basicola (Berk and Br.) Ferr. (David, 1978;Tsao and Bricker, 1966) and Ceratocystis wagnerii Goheen and Cobb (Hicks et al., 1980) proved useful for the isolation of T. paradoxa Since, standardization of an isolation method is a basic necessity for any study on plant pathogens, an attempt was made to improve upon existing methods.

References

1. ANON, 1976. Stern bleeding disease of coconut. CPCRI Annual Report CPCRI, Kasaragod, p. 62.

2. ANON, 1986. Stern bleeding disease of coconut. CPCRI Annual Report CPCRI, Kasaragod, p. 19.

3. DAVID, C.H. HSI. 1978. Effect of crop sequence, previous peanut blackhull severity, and time of sampling on soil population of Thielaviopsis basicola. Phypatholog 68: 1442 1445.

4. HICKS, B.R., COBB, F.W.R., AND GERSPER. P.L. 1980. Isolation of Ceratocystis wagener from forest soil with a selective m.edium. Phytopathology 70: 880 883.

5. MENON, K.P.V., and PANDALAI, K.M. 1960. The coconut palm A monograph. Published by Indian Central Coconut Committee p. 384.

6. NAMBIAR, K.K.N., JOSHI, Y., VENUGOPAL, M.N., and MOHAN, R.C. 1985. Stem bleeding disease of coconut: Reproduction of symptorns by inoculation with Thiela¬viopsis paradoxa. J. Plantation Crop 14: 130 133.

7. NAMBIAR, K.K.N., and SASTRY, R.K. 1988. Stern bleeding disease of coconut: Current status and approaches for its control. Philippine J. Coconut Studies 13(l) : 30 32.

8. OHLER, J.G. 1964. Coconut Tree of Life. Published by FAO. Rome. p. 446.

9. TSAO, P.H., and BRICKER, J.L. 1966. Chlamyclospores of Thielaviopsis basicola as surviving propagules in natural soils. Phytopathology 56(9): 1012 1014.
Published
1991-06-01
How to Cite
Anil Kumar, & K.K.N. Nambiar. (1991). AN IMPROVED METHOD FOR ISOLATION OF THIELAVIOPSIS PARADOXA FROM STEM BLEEDING AFFECTED COCONUT PALMS. CORD, 7(01), 34. https://doi.org/10.37833/cord.v7i01.243
Section
Articles