Optimization of Aqueous Extraction of Virgin Coconut Oil Using Response Surface Methodology

  • Ravindra Kumar Agarwal
  • S. John Don Bosco
Keywords: Virgin Coconut Oil, aqueous extraction process, optimization, response surface methodology


The present investigation deals with optimization of aqueous extraction process for maximum yield of virgin coconut oil (VCO) from coconut milk extracted from coconut meat.  Optimization of the process parameters including dilution ratio (w/w), grinding time (min), temperature (°C), cream separation time (hour) and pH was analyzed using one-factor-at-a-time (OFAT) method and response surface methodology based central composite design methods. OFAT method revealed the dilution ratio of 1:1 and grinding time of 3 min to enhance the yield of VCO whereas central composite design optimized the temperature at 33°C, cream separation time for 4 hours and pH of coconut milk at 6.5 for the maximum VCO yield of 20.38/100g of fresh coconut meat contained 32.82g/100g fat (w/w). Hence the percentage oil recovery of VCO was found to be 83.12 per cent (w/w) of fat present in fresh coconut meat. The analysis of variance (ANOVA) of central composite design revealed that temperature (P<0.05), pH (P<0.05) and interaction of these two parameters were highly significant for the yield of VCO with the second order model having the co-efficient of determination (R2) of 0.97.

Author Biography

S. John Don Bosco

Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India


AOAC Official methods of analysis. 1997. Association of Official Agricultural Chemist (17th ed.). Washington DC.
Cater, C.M., K.C. Rhee, R.D. Hagenmaier and K.F. Mattil. 1974. Aqueous extraction-an alternative oilseed milling. J. Am. Oil Chem. Soc. 51: 137-141.
Daniel, W.W. 1991. Biostatistics: A foundation for analysis in the health sciences. (5th ed.) Wiley: New York.
Embong, M.B. and P. Jelen. 1977. Technical feasibility of aqueous extraction of rapeseed oil-a laboratory study. J. Inst. Can. Sci. Technol. Aliment. 10: 239-243.
German, J.B. and C.J. Dillard. 2004. Saturated fats: what dietary intake? American Journal of Clinical Nutrition 80: 550-559.
Hagenmaier, R.D., C.M. Cater and K.F. Mattil. 1973. Aqueous processing of fresh Coconuts for recovery of oil and coconut skim milk. Journal of Food Science 38: 516-518.
Hagenmaier, R.D., C.M. Cater and K.F. Mattil. 1972. Critical unit operations of the aqueous processing of fresh coconuts. J. Am. Oil Chem. Soc. 49: 178-181.
Johnson, L.A. and E.W. Lusas. 1983. Comparison of alternative solvents for oils extraction. J. Am. Oil Chem. Soc. 60: 229-242.
Kim, H.K. 1989. Aqueous extraction of oil from palm kernel. J. Food Sci., 54: 491-492.
Lee, W.C., S. Yusof, N.S.A. Hamid, and B.S. Baharin. 2006. Optimizing conditions for Hot Water Extraction of banana juice using response surface methodology (RSM). Journal of Food Engineering 75 (4): 473-479.
Liu, S., F. Yang, C. Zhang, H. Ji, P. Hong and C. Deng. 2009. Optimization of process parameters for supercritical carbon dioxide extraction of Pass flora seed oil by response surface methodology. Journal of Supercritical Fluids 48: 9-14.
Madhavan, K., S.N. Kumar, and S. Azeez. 2005. Virgin coconut oil by fermentation method. Indian Coconut Journal 4:8-9.
Marina, A.M., Y.B. Che Man and I. Amin. 2009. Virgin coconut oil: emerging functional food oil. Trends Food Sci Technol. 20: 481-487.
Myers, R. and D.C. Montgomery. 2002. Response surface methodology. Wiley: New York, USA.
Nattapol, T. and J.N. Coupland. 2005. Effect of heating and homogenization on the stability of coconut milk emulsions. Journal of Food Science 70 (8): 466-470.
Nattapol, T. and J.N. Coupland. 2008. Effect of surface-active stabilizers on the microstructure and stability of coconut milk emulsions. Food Hydrocolloids 22: 1233-1242.
Nevin, K.G. and T. Rajamohan. 2004. Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clin Biochem 37: 830-835.
Onsaard, E., M. Vittayanont, S. Sringam, and D.J. Mc Clements. 2005. Properties and stability of oil-in-water emulsions stabilized by coconut skim milk proteins. J. Agric. Food Chemistry 53 (14): 5747-53.
Peamprasart, T., and N. Chiewchan. 2006. Effect of fat content and preheat treatment on the apparent viscosity of coconut milk after homogenization. Journal of Food Engineering 77: 653–658.
Rhee, K.C., C.M. Cater, and K.F. Mattil. 1972. Simultaneous recovery of protein and oil from raw peanuts in an aqueous system. J. Food Sci. 37: 90-93.
Rhee, K.C., Cater, C.M. and Mattil, K.F. 1973. Effect of processing pH on the properties of peanut protein isolates and oil. Cereal Chem. 50: 395-404.
Rosenthal, A., D.L. Pyle, and K. Niranjan. 1996. Aqueous and enzymatic processes for edible oil extraction. Enzyme Microbial Technology 19: 402-420.
Rustom, I.Y.S., M.H. Lopez-Leiva, and B.M. Nair. 1991. A study of factors affecting extraction of peanut (Arachis hypogaea L.) solids with water. Food Chemistry 42: 153-165.
US Department of Agriculture, Agricultural Research Service. 2001. USDA Nutrient Database for Standard Reference Release 14. US Department of Agriculture. USA.
Villarino, B.J., L.M. Dy, and M.C.C. Lizada. 2007. Descriptive sensory evaluation of virgin coconut oil and refined, bleached, deodorized coconut oil. Lebensmittel-Wissenschaft und Technologie (LWT) 40: 193-199.
How to Cite
Agarwal, R. K., & Bosco, S. J. D. (2013). Optimization of Aqueous Extraction of Virgin Coconut Oil Using Response Surface Methodology. CORD, 29(1), 35-45. https://doi.org/10.37833/cord.v29i1.96